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KMID : 0380020190340040248
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 4 p.248 ~ p.257
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Characterization of Eugenol an Inhibitory Material to Vaginitis-inducing Pathogen, Isolated from Syzygium aromaticum (L.) Merrill et Perry
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Park Sang-Hee
Ko Hee-Sun
Yoon Jae-Woo
Kim Hyun-Soo
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Abstract
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Vaginitis is a disease related to women's immunity and is largely divided into bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). In this study, we investigate Korean medicinal plants that have antimicrobial activity against vaginitis-inducing strains without inhibiting useful strains of bacteria. The antimicrobial activity results of the Syzygium aromaticum (L.) Merrill et Perry (SA) methyl alcohol extract were confirmed for C. albicans ATCC 28367, C. gleum KCTC 2904, and S. paucimobilis KCTC 2834 but not L. coleohominis KCTC 21007. Eugenol, a substance that shows antimicrobial activity, was separated from SA by reference to prior research. To study the characteristics of isolated eugenol from SA (IESA), we measured the antioxidant activity of IESA and stimulated vaginal epithelial cells (VK2/E6E7 ATCC CRL-2616) with LPS and C. albicans to confirm the effect of inhibiting stimulation. IESA showed higher antioxidant activity than commercial eugenol; the results of immunoblotting showed that IESA inhibited p38 activation at 60~90 minutes. According to the results of ELISA, the amount of TNF-α was 48.32% lower than that of C. albicans ATCC 28367 when treating together with IESA. Therefore, IESA could be developed as a therapeutic agent for BV and VVC.
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KEYWORD
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eugenol, Syzygium aromaticum (L.) Merrill et Perry, VK2/E6E7 ATCC CRL-2616, bacterial vaginosis, vulvovaginal candidiasis
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